High Throughput DNA Extraction
The DNA Factory has the capability to perform two different methods of 96-well format DNA purification. The difference between these two methods is the amount of final DNA concentration obtained, endotoxin purification capabilities, and overall cost.
| Standard Purity | High Purity | ||
|---|---|---|---|
| Validated Applications | Recombinational cloning | ✓ | ✓ |
| Restriction digests | ✓ | ✓ | |
| PCR templates | ✓ | ✓ | |
| Sanger Sequencing | ✓ | ✓ | |
| Next Generation Sequencing | ✓ | ✓ | |
| Ligations | ✓ | ✓ | |
| Transformations | ✓ | ✓ | |
| Transfections | - | ✓ | |
| DNA array printing | - | ✓ | |
| Protein expression | - | ✓ | |
| Endotoxin free | - | ✓ | |
| Turnaround time-bacterial pellet to DNA | 2 plates | 2 hours | 2 hours |
| 4 plates | 3.5 hours | 3.5 hours | |
| 2 plates normalized | - | 5.5 hours | |
| 4 plates normalized | - | 7.5 hours | |
| DNA Quality | OD 260/280 | 1.89±0.05 | 1.79±0.07 |
| DNA Quantity | Average Concentration |
250±14.1 ng/ul* (37.5ug) |
80±13.2ng/ul* (27ug) |
| Price | DNA prep | $125 | $325 |
| Representative Gel Image |  |
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DNA eluted in 150ul of water
DNA eluted in 300ul of elution buffer
